TransMed Research utilizes a large number of cell lines and primary cultures and employs a wide variety of approaches ranging from immunocytochemistry, biochemistry and molecular biology using disease-specific readouts. The in vitro facility is GLP-accredited and validations are performed in accordance with OECD and FDA guidelines.
Cell systems
- cell lines (including SH-SY5Y, NT2D1, PC12, Murine Embryonic Fibroblasts)
- primary cultures (including rat/mouse primary cortical neurons)
- stem cells (rat embryonic stem cells, rat/mouse embryonic/adult neural stem cells, animal and human mesenchymal stem cells)
Cell assays
- viability (MTT, Neutral Red, pycnotic nuclei analysis, mithocondrial integrity)
- neuronal profiling (neurites maturation and elongation)
- stem cell lineage (immunocytochemistry/molecular biology)
Imaging
- conventional microscopy and fluorescence microscopy
- confocal time lapse microscopy
- 2D, 3D, 4D-computerized image analysis